Construct: shRNA TRCN0000267616

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001033261.2-5245s21c1
DNA Barcode:
ACCCTCAGAATGCGGAAATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zfc3h1 (216345)
Gene Description:
zinc finger, C3H1-type containing
Transcript:
RefSeq NM_001033261.2 (CURRENT)
Match location:
Position 5245 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000267616)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCCTCAGAATGCGGAAATTT
Hairpin Sequence:
5'-CCGG-ACCCTCAGAATGCGGAAATTT-CTCGAG-AAATTTCCGCATTCTGAGGGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCCTCAGAATGCGGAAATTTCTCGAGAAATTTCCGCATTCTGAGGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCCTCAGAATGCGGAAATTTCTCGAGAAATTTCCGCATTCTGAGGGT-3'

Other clones with same target sequence:

(none)