Construct: shRNA TRCN0000267620

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172268.2-8013s21c1
DNA Barcode:
GATCCACCCTTTCCGATATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nup214 (227720)
Gene Description:
nucleoporin 214
Transcript:
RefSeq NM_172268.2 (CURRENT)
Match location:
Position 8013 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000267620)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATCCACCCTTTCCGATATAT
Hairpin Sequence:
5'-CCGG-GATCCACCCTTTCCGATATAT-CTCGAG-ATATATCGGAAAGGGTGGATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATCCACCCTTTCCGATATATCTCGAGATATATCGGAAAGGGTGGATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATCCACCCTTTCCGATATATCTCGAGATATATCGGAAAGGGTGGATC-3'

Other clones with same target sequence:

(none)