Construct: shRNA TRCN0000267804

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001081227.1-445s21c1
DNA Barcode:
CTGCGTCTTCTGGCTGAATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Stum (381310)
Gene Description:
mechanosensory transduction mediator
Transcript:
RefSeq NM_001081227.1 (NON-CURRENT)
Match location:
Position 445 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000267804)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGCGTCTTCTGGCTGAATAT
Hairpin Sequence:
5'-CCGG-CTGCGTCTTCTGGCTGAATAT-CTCGAG-ATATTCAGCCAGAAGACGCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGCGTCTTCTGGCTGAATATCTCGAGATATTCAGCCAGAAGACGCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGCGTCTTCTGGCTGAATATCTCGAGATATTCAGCCAGAAGACGCAG-3'

Other clones with same target sequence:

(none)