Construct: shRNA TRCN0000267957

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001081351.1-2245s21c1
DNA Barcode:
CGAGGAAATTCACTCAATTAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cped1 (214642)
Gene Description:
cadherin-like and PC-esterase domain containing 1
Transcript:
RefSeq NM_001081351.1 (CURRENT)
Match location:
Position 2245 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000267957)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGGAAATTCACTCAATTAG
Hairpin Sequence:
5'-CCGG-CGAGGAAATTCACTCAATTAG-CTCGAG-CTAATTGAGTGAATTTCCTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGGAAATTCACTCAATTAGCTCGAGCTAATTGAGTGAATTTCCTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGGAAATTCACTCAATTAGCTCGAGCTAATTGAGTGAATTTCCTCG-3'

Other clones with same target sequence:

(none)