Construct: shRNA TRCN0000268110

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001126487.1-2185s21c1
DNA Barcode:
CTGCGTGTCATTGCGGAATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mroh7 (381538)
Gene Description:
maestro heat-like repeat family member 7
Transcript:
RefSeq NM_001126487.1 (CURRENT)
Match location:
Position 2185 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000268110)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGCGTGTCATTGCGGAATTT
Hairpin Sequence:
5'-CCGG-CTGCGTGTCATTGCGGAATTT-CTCGAG-AAATTCCGCAATGACACGCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGCGTGTCATTGCGGAATTTCTCGAGAAATTCCGCAATGACACGCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGCGTGTCATTGCGGAATTTCTCGAGAAATTCCGCAATGACACGCAG-3'

Other clones with same target sequence:

(none)