Construct: shRNA TRCN0000268130

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001122663.1-2153s21c1
DNA Barcode:
TCCTGGTGACCTTGAACTATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
C030017K20Rik (381581)
Gene Description:
RIKEN cDNA C030017K20 gene
Transcript:
RefSeq NM_001122663.1 (NON-CURRENT)
Match location:
Position 2153 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000268130)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCTGGTGACCTTGAACTATG
Hairpin Sequence:
5'-CCGG-TCCTGGTGACCTTGAACTATG-CTCGAG-CATAGTTCAAGGTCACCAGGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCTGGTGACCTTGAACTATGCTCGAGCATAGTTCAAGGTCACCAGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCTGGTGACCTTGAACTATGCTCGAGCATAGTTCAAGGTCACCAGGA-3'

Other clones with same target sequence:

(none)