Construct: shRNA TRCN0000268218

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001013778.1-636s21c1
DNA Barcode:
TGGATATTTGAAGCCAGAAAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tvp23a (383103)
Gene Description:
trans-golgi network vesicle protein 23A
Transcript:
RefSeq NM_001013778.1 (CURRENT)
Match location:
Position 636 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000268218)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGATATTTGAAGCCAGAAAG
Hairpin Sequence:
5'-CCGG-TGGATATTTGAAGCCAGAAAG-CTCGAG-CTTTCTGGCTTCAAATATCCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGATATTTGAAGCCAGAAAGCTCGAGCTTTCTGGCTTCAAATATCCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGATATTTGAAGCCAGAAAGCTCGAGCTTTCTGGCTTCAAATATCCA-3'

Other clones with same target sequence:

(none)