Construct: shRNA TRCN0000268371

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_183116.2-381s21c1
DNA Barcode:
TTCGGATGCTATGCGTTATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc18b1 (76306)
Gene Description:
solute carrier family 18, subfamily B, member 1
Transcript:
RefSeq NM_183116.2 (CURRENT)
Match location:
Position 381 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000268371)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTCGGATGCTATGCGTTATTT
Hairpin Sequence:
5'-CCGG-TTCGGATGCTATGCGTTATTT-CTCGAG-AAATAACGCATAGCATCCGAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTCGGATGCTATGCGTTATTTCTCGAGAAATAACGCATAGCATCCGAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTCGGATGCTATGCGTTATTTCTCGAGAAATAACGCATAGCATCCGAA-3'

Other clones with same target sequence:

(none)