Construct: shRNA TRCN0000268408

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020864.1-2116s21c1
DNA Barcode:
ATCGTTAGCCAATCGTGATTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NYAP2 (57624)
Gene Description:
neuronal tyrosine-phosphorylated phosphoinositide-3-kinase adaptor 2
Transcript:
RefSeq NM_020864.1 (CURRENT)
Match location:
Position 2116 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000268408)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATCGTTAGCCAATCGTGATTG
Hairpin Sequence:
5'-CCGG-ATCGTTAGCCAATCGTGATTG-CTCGAG-CAATCACGATTGGCTAACGAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATCGTTAGCCAATCGTGATTGCTCGAGCAATCACGATTGGCTAACGATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATCGTTAGCCAATCGTGATTGCTCGAGCAATCACGATTGGCTAACGAT-3'

Other clones with same target sequence:

(none)