Construct: shRNA TRCN0000268504

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001012971.3-436s21c1
DNA Barcode:
TCGAGGTGGAGCTTATGAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FAM209A (200232)
Gene Description:
family with sequence similarity 209 member A
Transcript:
RefSeq NM_001012971.3 (CURRENT)
Match location:
Position 436 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000268504)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCGAGGTGGAGCTTATGAAAT
Hairpin Sequence:
5'-CCGG-TCGAGGTGGAGCTTATGAAAT-CTCGAG-ATTTCATAAGCTCCACCTCGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCGAGGTGGAGCTTATGAAATCTCGAGATTTCATAAGCTCCACCTCGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCGAGGTGGAGCTTATGAAATCTCGAGATTTCATAAGCTCCACCTCGA-3'

Other clones with same target sequence:

(none)