Construct: shRNA TRCN0000268724

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027001.1-1917s21c1
DNA Barcode:
GCCCACTTCATGGCAATATTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pdzph1 (69239)
Gene Description:
PDZ and pleckstrin homology domains 1
Transcript:
RefSeq NM_027001.1 (CURRENT)
Match location:
Position 1917 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000268724)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCACTTCATGGCAATATTC
Hairpin Sequence:
5'-CCGG-GCCCACTTCATGGCAATATTC-CTCGAG-GAATATTGCCATGAAGTGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCACTTCATGGCAATATTCCTCGAGGAATATTGCCATGAAGTGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCACTTCATGGCAATATTCCTCGAGGAATATTGCCATGAAGTGGGC-3'

Other clones with same target sequence:

(none)