Construct: shRNA TRCN0000268743

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177608.3-1386s21c1
DNA Barcode:
GACCCAAATTCCGAGTCTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Secisbp2l (70354)
Gene Description:
SECIS binding protein 2-like
Transcript:
RefSeq NM_177608.3 (CURRENT)
Match location:
Position 1386 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000268743)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACCCAAATTCCGAGTCTTTA
Hairpin Sequence:
5'-CCGG-GACCCAAATTCCGAGTCTTTA-CTCGAG-TAAAGACTCGGAATTTGGGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACCCAAATTCCGAGTCTTTACTCGAGTAAAGACTCGGAATTTGGGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACCCAAATTCCGAGTCTTTACTCGAGTAAAGACTCGGAATTTGGGTC-3'

Other clones with same target sequence:

(none)