Construct: shRNA TRCN0000268775

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001001986.1-232s21c1
DNA Barcode:
GGTGTCTGGCATTATTGATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nol4l (329540)
Gene Description:
nucleolar protein 4-like
Transcript:
RefSeq NM_001001986.1 (NON-CURRENT)
Match location:
Position 232 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000268775)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGTGTCTGGCATTATTGATTA
Hairpin Sequence:
5'-CCGG-GGTGTCTGGCATTATTGATTA-CTCGAG-TAATCAATAATGCCAGACACC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGTGTCTGGCATTATTGATTACTCGAGTAATCAATAATGCCAGACACCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGTGTCTGGCATTATTGATTACTCGAGTAATCAATAATGCCAGACACC-3'

Other clones with same target sequence:

(none)