Construct: shRNA TRCN0000268777

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001004187.1-273s21c1
DNA Barcode:
ATGGACCTCCTTCGCATATTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rexo5 (434234)
Gene Description:
RNA exonuclease 5
Transcript:
RefSeq NM_001004187.1 (CURRENT)
Match location:
Position 273 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000268777)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGGACCTCCTTCGCATATTC
Hairpin Sequence:
5'-CCGG-ATGGACCTCCTTCGCATATTC-CTCGAG-GAATATGCGAAGGAGGTCCAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGGACCTCCTTCGCATATTCCTCGAGGAATATGCGAAGGAGGTCCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGGACCTCCTTCGCATATTCCTCGAGGAATATGCGAAGGAGGTCCAT-3'

Other clones with same target sequence:

(none)