Construct: shRNA TRCN0000268791

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177608.3-917s21c1
DNA Barcode:
CCCATCAGACATCGCTAATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Secisbp2l (70354)
Gene Description:
SECIS binding protein 2-like
Transcript:
RefSeq NM_177608.3 (CURRENT)
Match location:
Position 917 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000268791)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCATCAGACATCGCTAATAA
Hairpin Sequence:
5'-CCGG-CCCATCAGACATCGCTAATAA-CTCGAG-TTATTAGCGATGTCTGATGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCATCAGACATCGCTAATAACTCGAGTTATTAGCGATGTCTGATGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCATCAGACATCGCTAATAACTCGAGTTATTAGCGATGTCTGATGGG-3'

Other clones with same target sequence:

(none)