Construct: shRNA TRCN0000268941

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001081365.1-394s21c1
DNA Barcode:
GACCTCAGGAGCCTCCTAAGA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cystm1 (66060)
Gene Description:
cysteine-rich transmembrane module containing 1
Transcript:
RefSeq NM_001081365.1 (CURRENT)
Match location:
Position 394 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000268941)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACCTCAGGAGCCTCCTAAGA
Hairpin Sequence:
5'-CCGG-GACCTCAGGAGCCTCCTAAGA-CTCGAG-TCTTAGGAGGCTCCTGAGGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACCTCAGGAGCCTCCTAAGACTCGAGTCTTAGGAGGCTCCTGAGGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACCTCAGGAGCCTCCTAAGACTCGAGTCTTAGGAGGCTCCTGAGGTC-3'

Other clones with same target sequence:

(none)