Construct: shRNA TRCN0000269328

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001033176.1-577s21c1
DNA Barcode:
CCCATGGTGCTCATCATAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc6a21 (76713)
Gene Description:
solute carrier family 6 member 21
Transcript:
RefSeq NM_001033176.1 (CURRENT)
Match location:
Position 577 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000269328)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCATGGTGCTCATCATAATT
Hairpin Sequence:
5'-CCGG-CCCATGGTGCTCATCATAATT-CTCGAG-AATTATGATGAGCACCATGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCATGGTGCTCATCATAATTCTCGAGAATTATGATGAGCACCATGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCATGGTGCTCATCATAATTCTCGAGAATTATGATGAGCACCATGGG-3'

Other clones with same target sequence:

(none)