Construct: shRNA TRCN0000269501

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001100878.1-675s21c1
DNA Barcode:
AGCCGTAACCAGCGTGTAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MROH6 (642475)
Gene Description:
maestro heat like repeat family member 6
Transcript:
RefSeq NM_001100878.1 (NON-CURRENT)
Match location:
Position 675 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000269501)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCCGTAACCAGCGTGTAAAT
Hairpin Sequence:
5'-CCGG-AGCCGTAACCAGCGTGTAAAT-CTCGAG-ATTTACACGCTGGTTACGGCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCCGTAACCAGCGTGTAAATCTCGAGATTTACACGCTGGTTACGGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCCGTAACCAGCGTGTAAATCTCGAGATTTACACGCTGGTTACGGCT-3'

Other clones with same target sequence:

(none)