Construct: shRNA TRCN0000269513

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001033180.2-1409s21c1
DNA Barcode:
GAAGCGCTGTTGAACATTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Axdnd1 (77352)
Gene Description:
axonemal dynein light chain domain containing 1
Transcript:
RefSeq NM_001033180.2 (NON-CURRENT)
Match location:
Position 1409 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000269513)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGCGCTGTTGAACATTATT
Hairpin Sequence:
5'-CCGG-GAAGCGCTGTTGAACATTATT-CTCGAG-AATAATGTTCAACAGCGCTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGCGCTGTTGAACATTATTCTCGAGAATAATGTTCAACAGCGCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGCGCTGTTGAACATTATTCTCGAGAATAATGTTCAACAGCGCTTC-3'

Other clones with same target sequence:

(none)