Construct: shRNA TRCN0000269630

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001123225.1-259s21c1
DNA Barcode:
ATGGAGAAGAACTGGCAAGAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SYCE3 (644186)
Gene Description:
synaptonemal complex central element protein 3
Transcript:
RefSeq NM_001123225.1 (NON-CURRENT)
Match location:
Position 259 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000269630)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGGAGAAGAACTGGCAAGAG
Hairpin Sequence:
5'-CCGG-ATGGAGAAGAACTGGCAAGAG-CTCGAG-CTCTTGCCAGTTCTTCTCCAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGGAGAAGAACTGGCAAGAGCTCGAGCTCTTGCCAGTTCTTCTCCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGGAGAAGAACTGGCAAGAGCTCGAGCTCTTGCCAGTTCTTCTCCAT-3'

Other clones with same target sequence:

(none)