Construct: shRNA TRCN0000269660

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001083124.1-1459s21c1
DNA Barcode:
CCGAGTGCCAACCCTTTATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SPATA31A3 (727830)
Gene Description:
SPATA31 subfamily A member 3
Transcript:
RefSeq NM_001083124.1 (CURRENT)
Match location:
Position 1459 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000269660)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGAGTGCCAACCCTTTATTT
Hairpin Sequence:
5'-CCGG-CCGAGTGCCAACCCTTTATTT-CTCGAG-AAATAAAGGGTTGGCACTCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGAGTGCCAACCCTTTATTTCTCGAGAAATAAAGGGTTGGCACTCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGAGTGCCAACCCTTTATTTCTCGAGAAATAAAGGGTTGGCACTCGG-3'

Other clones with same target sequence:

(none)