Construct: shRNA TRCN0000269678

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001126062.1-226s21c1
DNA Barcode:
GGAACCTGGCTTACCAGTATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TPTE2P1 (646405)
Gene Description:
transmembrane phosphoinositide 3-phosphatase and tensin homolog 2 pseudogene 1
Transcript:
RefSeq NM_001126062.1 (NON-CURRENT)
Match location:
Position 226 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000269678)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGAACCTGGCTTACCAGTATA
Hairpin Sequence:
5'-CCGG-GGAACCTGGCTTACCAGTATA-CTCGAG-TATACTGGTAAGCCAGGTTCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGAACCTGGCTTACCAGTATACTCGAGTATACTGGTAAGCCAGGTTCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGAACCTGGCTTACCAGTATACTCGAGTATACTGGTAAGCCAGGTTCC-3'

Other clones with same target sequence:

(none)