Construct: shRNA TRCN0000269681

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001098843.3-1596s21c1
DNA Barcode:
GGGAATCGACAGCGAAGAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CXorf30 (645090)
Gene Description:
chromosome X open reading frame 30
Transcript:
RefSeq NM_001098843.3 (NON-CURRENT)
Match location:
Position 1596 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000269681)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGGAATCGACAGCGAAGAAAT
Hairpin Sequence:
5'-CCGG-GGGAATCGACAGCGAAGAAAT-CTCGAG-ATTTCTTCGCTGTCGATTCCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGGAATCGACAGCGAAGAAATCTCGAGATTTCTTCGCTGTCGATTCCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGGAATCGACAGCGAAGAAATCTCGAGATTTCTTCGCTGTCGATTCCC-3'

Other clones with same target sequence:

(none)