Construct: shRNA TRCN0000269765

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001113541.1-3656s21c1
DNA Barcode:
CATGACGGCAGTTGGACAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SPATA31A5 (727905)
Gene Description:
SPATA31 subfamily A member 5
Transcript:
RefSeq NM_001113541.1 (CURRENT)
Match location:
Position 3656 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000269765)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATGACGGCAGTTGGACAAAT
Hairpin Sequence:
5'-CCGG-CATGACGGCAGTTGGACAAAT-CTCGAG-ATTTGTCCAACTGCCGTCATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATGACGGCAGTTGGACAAATCTCGAGATTTGTCCAACTGCCGTCATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATGACGGCAGTTGGACAAATCTCGAGATTTGTCCAACTGCCGTCATG-3'

Other clones with same target sequence:

(none)