Construct: shRNA TRCN0000270018

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001123370.1-986s21c1
DNA Barcode:
CCGAGTCAACAATTCTATATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
9030025P20Rik (100041574)
Gene Description:
RIKEN cDNA 9030025P20 gene
Transcript:
RefSeq NM_001123370.1 (CURRENT)
Match location:
Position 986 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000270018)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGAGTCAACAATTCTATATA
Hairpin Sequence:
5'-CCGG-CCGAGTCAACAATTCTATATA-CTCGAG-TATATAGAATTGTTGACTCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGAGTCAACAATTCTATATACTCGAGTATATAGAATTGTTGACTCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGAGTCAACAATTCTATATACTCGAGTATATAGAATTGTTGACTCGG-3'

Other clones with same target sequence:

(none)