Construct: shRNA TRCN0000270073

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001099301.1-383s21c1
DNA Barcode:
AGGGCTACTACATCTTGTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Obp2b (383678)
Gene Description:
odorant binding protein 2B
Transcript:
RefSeq NM_001099301.1 (CURRENT)
Match location:
Position 383 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000270073)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGGCTACTACATCTTGTATT
Hairpin Sequence:
5'-CCGG-AGGGCTACTACATCTTGTATT-CTCGAG-AATACAAGATGTAGTAGCCCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGGCTACTACATCTTGTATTCTCGAGAATACAAGATGTAGTAGCCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGGCTACTACATCTTGTATTCTCGAGAATACAAGATGTAGTAGCCCT-3'

Other clones with same target sequence:

(none)