Construct: shRNA TRCN0000270393

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001100444.1-2321s21c1
DNA Barcode:
TTCTCACTTGCTGGTACATTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm5934 (546272)
Gene Description:
predicted gene 5934
Transcript:
RefSeq NM_001100444.1 (NON-CURRENT)
Match location:
Position 2321 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000270393)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTCTCACTTGCTGGTACATTG
Hairpin Sequence:
5'-CCGG-TTCTCACTTGCTGGTACATTG-CTCGAG-CAATGTACCAGCAAGTGAGAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTCTCACTTGCTGGTACATTGCTCGAGCAATGTACCAGCAAGTGAGAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTCTCACTTGCTGGTACATTGCTCGAGCAATGTACCAGCAAGTGAGAA-3'

Other clones with same target sequence:

(none)