Construct: shRNA TRCN0000270399

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001085530.1-3843s21c1
DNA Barcode:
TGTCTAACTCCTTCCTATATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fam205a2 (545611)
Gene Description:
family with sequence similarity 205, member A2
Transcript:
RefSeq NM_001085530.1 (CURRENT)
Match location:
Position 3843 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000270399)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGTCTAACTCCTTCCTATATT
Hairpin Sequence:
5'-CCGG-TGTCTAACTCCTTCCTATATT-CTCGAG-AATATAGGAAGGAGTTAGACA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGTCTAACTCCTTCCTATATTCTCGAGAATATAGGAAGGAGTTAGACATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGTCTAACTCCTTCCTATATTCTCGAGAATATAGGAAGGAGTTAGACA-3'

Other clones with same target sequence:

(none)