Construct: shRNA TRCN0000270529

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001039222.1-327s21c1
DNA Barcode:
CAACAATAATTGAACGCATGA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Clca4c-ps (622139)
Gene Description:
chloride channel accessory 4C, pseudogene
Transcript:
RefSeq NM_001039222.1 (NON-CURRENT)
Match location:
Position 327 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000270529)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAACAATAATTGAACGCATGA
Hairpin Sequence:
5'-CCGG-CAACAATAATTGAACGCATGA-CTCGAG-TCATGCGTTCAATTATTGTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAACAATAATTGAACGCATGACTCGAGTCATGCGTTCAATTATTGTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAACAATAATTGAACGCATGACTCGAGTCATGCGTTCAATTATTGTTG-3'

Other clones with same target sequence:

(none)