Construct: shRNA TRCN0000270588

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001039250.1-758s21c1
DNA Barcode:
GTGTTTATTTAGTTGTGTTTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm7247 (638695)
Gene Description:
predicted gene 7247
Transcript:
RefSeq NM_001039250.1 (NON-CURRENT)
Match location:
Position 758 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000270588)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGTTTATTTAGTTGTGTTTG
Hairpin Sequence:
5'-CCGG-GTGTTTATTTAGTTGTGTTTG-CTCGAG-CAAACACAACTAAATAAACAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGTTTATTTAGTTGTGTTTGCTCGAGCAAACACAACTAAATAAACACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGTTTATTTAGTTGTGTTTGCTCGAGCAAACACAACTAAATAAACAC-3'

Other clones with same target sequence:

(none)