Construct: shRNA TRCN0000270615

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001039219.2-3567s21c1
DNA Barcode:
TAGTTGGACCTTGGTAGTTAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gal3st2c (619597)
Gene Description:
galactose-3-O-sulfotransferase 2C
Transcript:
RefSeq NM_001039219.2 (CURRENT)
Match location:
Position 3567 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000270615)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAGTTGGACCTTGGTAGTTAG
Hairpin Sequence:
5'-CCGG-TAGTTGGACCTTGGTAGTTAG-CTCGAG-CTAACTACCAAGGTCCAACTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAGTTGGACCTTGGTAGTTAGCTCGAGCTAACTACCAAGGTCCAACTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAGTTGGACCTTGGTAGTTAGCTCGAGCTAACTACCAAGGTCCAACTA-3'

Other clones with same target sequence:

(none)