Construct: shRNA TRCN0000270795

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001081325.1-335s21c1
DNA Barcode:
CCAAAGCTAAGGTGATCTATC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sult2a6 (629219)
Gene Description:
sulfotransferase family 2A, dehydroepiandrosterone (DHEA)-preferring, member 6
Transcript:
RefSeq NM_001081325.1 (NON-CURRENT)
Match location:
Position 335 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000270795)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAAGCTAAGGTGATCTATC
Hairpin Sequence:
5'-CCGG-CCAAAGCTAAGGTGATCTATC-CTCGAG-GATAGATCACCTTAGCTTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAAGCTAAGGTGATCTATCCTCGAGGATAGATCACCTTAGCTTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAAGCTAAGGTGATCTATCCTCGAGGATAGATCACCTTAGCTTTGG-3'

Other clones with same target sequence:

(none)