Construct: shRNA TRCN0000270833
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_001081284.1-561s21c1
- DNA Barcode:
- ACGCCCATTGAGCTCCAATTT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Gm12695 (620779)
- Gene Description:
- predicted gene 12695
- Transcript:
- RefSeq NM_001081284.1 (CURRENT)
- Match location:
- Position 561 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- ACGCCCATTGAGCTCCAATTT
- Hairpin Sequence:
-
5'-CCGG-ACGCCCATTGAGCTCCAATTT-CTCGAG-AAATTGGAGCTCAATGGGCGT-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGACGCCCATTGAGCTCCAATTTCTCGAGAAATTGGAGCTCAATGGGCGTTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAACGCCCATTGAGCTCCAATTTCTCGAGAAATTGGAGCTCAATGGGCGT-3'
Other clones with same target sequence:
(none)
