Construct: shRNA TRCN0000271228

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000861.3-3815s21c1
DNA Barcode:
AGTGGCTAAGTGTCCATTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HRH1 (3269)
Gene Description:
histamine receptor H1
Transcript:
RefSeq NM_000861.3 (CURRENT)
Match location:
Position 3815 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000271228)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTGGCTAAGTGTCCATTATT
Hairpin Sequence:
5'-CCGG-AGTGGCTAAGTGTCCATTATT-CTCGAG-AATAATGGACACTTAGCCACT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTGGCTAAGTGTCCATTATTCTCGAGAATAATGGACACTTAGCCACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTGGCTAAGTGTCCATTATTCTCGAGAATAATGGACACTTAGCCACT-3'

Other clones with same target sequence:

(none)