Construct: shRNA TRCN0000271247

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004904.2-656s21c1
DNA Barcode:
GATGCCAATGGAGCGACAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CREB5 (9586)
Gene Description:
cAMP responsive element binding protein 5
Transcript:
RefSeq NM_004904.2 (NON-CURRENT)
Match location:
Position 656 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000271247)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGCCAATGGAGCGACAAAT
Hairpin Sequence:
5'-CCGG-GATGCCAATGGAGCGACAAAT-CTCGAG-ATTTGTCGCTCCATTGGCATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGCCAATGGAGCGACAAATCTCGAGATTTGTCGCTCCATTGGCATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGCCAATGGAGCGACAAATCTCGAGATTTGTCGCTCCATTGGCATC-3'

Other clones with same target sequence:

(none)