Construct: shRNA TRCN0000271274

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005605.3-1880s21c1
DNA Barcode:
CGTGGCTCAGGTGGATCTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PPP3CC (5533)
Gene Description:
protein phosphatase 3 catalytic subunit gamma
Transcript:
RefSeq NM_005605.3 (NON-CURRENT)
Match location:
Position 1880 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000271274)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTGGCTCAGGTGGATCTAAA
Hairpin Sequence:
5'-CCGG-CGTGGCTCAGGTGGATCTAAA-CTCGAG-TTTAGATCCACCTGAGCCACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTGGCTCAGGTGGATCTAAACTCGAGTTTAGATCCACCTGAGCCACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTGGCTCAGGTGGATCTAAACTCGAGTTTAGATCCACCTGAGCCACG-3'

Other clones with same target sequence:

(none)