Construct: shRNA TRCN0000271277

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000222.2-2942s21c1
DNA Barcode:
ACCATTCTGTGCGGATCAATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KIT (3815)
Gene Description:
KIT proto-oncogene, receptor tyrosine kinase
Transcript:
RefSeq NM_000222.2 (CURRENT)
Match location:
Position 2942 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000271277)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCATTCTGTGCGGATCAATT
Hairpin Sequence:
5'-CCGG-ACCATTCTGTGCGGATCAATT-CTCGAG-AATTGATCCGCACAGAATGGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCATTCTGTGCGGATCAATTCTCGAGAATTGATCCGCACAGAATGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCATTCTGTGCGGATCAATTCTCGAGAATTGATCCGCACAGAATGGT-3'

Other clones with same target sequence:

(none)