Construct: shRNA TRCN0000271314

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020652.2-774s21c1
DNA Barcode:
ACCATTGAAGCTTGAGAGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF286A (57335)
Gene Description:
zinc finger protein 286A
Transcript:
RefSeq NM_020652.2 (NON-CURRENT)
Match location:
Position 774 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000271314)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCATTGAAGCTTGAGAGAAA
Hairpin Sequence:
5'-CCGG-ACCATTGAAGCTTGAGAGAAA-CTCGAG-TTTCTCTCAAGCTTCAATGGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCATTGAAGCTTGAGAGAAACTCGAGTTTCTCTCAAGCTTCAATGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCATTGAAGCTTGAGAGAAACTCGAGTTTCTCTCAAGCTTCAATGGT-3'

Other clones with same target sequence:

(none)