Construct: shRNA TRCN0000271319

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001171.5-4628s21c1
DNA Barcode:
TCGATAGCTCCACACGATATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ABCC6 (368)
Gene Description:
ATP binding cassette subfamily C member 6
Transcript:
RefSeq NM_001171.5 (CURRENT)
Match location:
Position 4628 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000271319)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCGATAGCTCCACACGATATT
Hairpin Sequence:
5'-CCGG-TCGATAGCTCCACACGATATT-CTCGAG-AATATCGTGTGGAGCTATCGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCGATAGCTCCACACGATATTCTCGAGAATATCGTGTGGAGCTATCGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCGATAGCTCCACACGATATTCTCGAGAATATCGTGTGGAGCTATCGA-3'

Other clones with same target sequence:

(none)