Construct: shRNA TRCN0000271344

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019057.3-4006s21c1
DNA Barcode:
TGCCAGCTGAAGGCGTATAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FAM193B (54540)
Gene Description:
family with sequence similarity 193 member B
Transcript:
RefSeq NM_019057.3 (NON-CURRENT)
Match location:
Position 4006 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000271344)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCCAGCTGAAGGCGTATAAT
Hairpin Sequence:
5'-CCGG-TGCCAGCTGAAGGCGTATAAT-CTCGAG-ATTATACGCCTTCAGCTGGCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCCAGCTGAAGGCGTATAATCTCGAGATTATACGCCTTCAGCTGGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCCAGCTGAAGGCGTATAATCTCGAGATTATACGCCTTCAGCTGGCA-3'

Other clones with same target sequence:

(none)