Construct: shRNA TRCN0000271350

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_134424.2-454s21c1
DNA Barcode:
CAGCTGAAGGATGGTTCATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RAD52 (5893)
Gene Description:
RAD52 homolog, DNA repair protein
Transcript:
RefSeq NM_134424.2 (NON-CURRENT)
Match location:
Position 454 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000271350)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGCTGAAGGATGGTTCATAT
Hairpin Sequence:
5'-CCGG-CAGCTGAAGGATGGTTCATAT-CTCGAG-ATATGAACCATCCTTCAGCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGCTGAAGGATGGTTCATATCTCGAGATATGAACCATCCTTCAGCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGCTGAAGGATGGTTCATATCTCGAGATATGAACCATCCTTCAGCTG-3'

Other clones with same target sequence:

(none)