Construct: shRNA TRCN0000271401

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018593.3-2173s21c1
DNA Barcode:
TACCTAAACCTCAAGTCTATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC16A10 (117247)
Gene Description:
solute carrier family 16 member 10
Transcript:
RefSeq NM_018593.3 (NON-CURRENT)
Match location:
Position 2173 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000271401)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TACCTAAACCTCAAGTCTATG
Hairpin Sequence:
5'-CCGG-TACCTAAACCTCAAGTCTATG-CTCGAG-CATAGACTTGAGGTTTAGGTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTACCTAAACCTCAAGTCTATGCTCGAGCATAGACTTGAGGTTTAGGTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATACCTAAACCTCAAGTCTATGCTCGAGCATAGACTTGAGGTTTAGGTA-3'

Other clones with same target sequence:

(none)