Construct: shRNA TRCN0000271511

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014388.5-1945s21c1
DNA Barcode:
CTTCGTGCGTCTTCGAAATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
UTP25 (27042)
Gene Description:
UTP25 small subunit processor component
Transcript:
RefSeq NM_014388.5 (NON-CURRENT)
Match location:
Position 1945 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000271511)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTCGTGCGTCTTCGAAATTA
Hairpin Sequence:
5'-CCGG-CTTCGTGCGTCTTCGAAATTA-CTCGAG-TAATTTCGAAGACGCACGAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTCGTGCGTCTTCGAAATTACTCGAGTAATTTCGAAGACGCACGAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTCGTGCGTCTTCGAAATTACTCGAGTAATTTCGAAGACGCACGAAG-3'

Other clones with same target sequence:

(none)