Construct: shRNA TRCN0000271528

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006785.2-1542s21c1
DNA Barcode:
TGATGCTCCAAATCCATATAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MALT1 (10892)
Gene Description:
MALT1 paracaspase
Transcript:
RefSeq NM_006785.2 (NON-CURRENT)
Match location:
Position 1542 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000271528)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGATGCTCCAAATCCATATAG
Hairpin Sequence:
5'-CCGG-TGATGCTCCAAATCCATATAG-CTCGAG-CTATATGGATTTGGAGCATCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGATGCTCCAAATCCATATAGCTCGAGCTATATGGATTTGGAGCATCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGATGCTCCAAATCCATATAGCTCGAGCTATATGGATTTGGAGCATCA-3'

Other clones with same target sequence:

  1. TRCN0000364270