Construct: shRNA TRCN0000271603

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001126339.1-763s21c1
DNA Barcode:
GCTCTTGGGAGCTGATGAAGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
THTPA (79178)
Gene Description:
thiamine triphosphatase
Transcript:
RefSeq NM_001126339.1 (NON-CURRENT)
Match location:
Position 763 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000271603)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCTTGGGAGCTGATGAAGA
Hairpin Sequence:
5'-CCGG-GCTCTTGGGAGCTGATGAAGA-CTCGAG-TCTTCATCAGCTCCCAAGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCTTGGGAGCTGATGAAGACTCGAGTCTTCATCAGCTCCCAAGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCTTGGGAGCTGATGAAGACTCGAGTCTTCATCAGCTCCCAAGAGC-3'

Other clones with same target sequence:

(none)