Construct: shRNA TRCN0000271655

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014853.2-1372s21c1
DNA Barcode:
AGCATTCGCTCCGTGGATATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SGSM2 (9905)
Gene Description:
small G protein signaling modulator 2
Transcript:
RefSeq NM_014853.2 (NON-CURRENT)
Match location:
Position 1372 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000271655)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCATTCGCTCCGTGGATATG
Hairpin Sequence:
5'-CCGG-AGCATTCGCTCCGTGGATATG-CTCGAG-CATATCCACGGAGCGAATGCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCATTCGCTCCGTGGATATGCTCGAGCATATCCACGGAGCGAATGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCATTCGCTCCGTGGATATGCTCGAGCATATCCACGGAGCGAATGCT-3'

Other clones with same target sequence:

(none)