Construct: shRNA TRCN0000271792

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172647.2-901s21c1
DNA Barcode:
CACCGGGTAAGAAGGTCATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
F11r (16456)
Gene Description:
F11 receptor
Transcript:
RefSeq NM_172647.2 (CURRENT)
Match location:
Position 901 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000271792)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACCGGGTAAGAAGGTCATTT
Hairpin Sequence:
5'-CCGG-CACCGGGTAAGAAGGTCATTT-CTCGAG-AAATGACCTTCTTACCCGGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACCGGGTAAGAAGGTCATTTCTCGAGAAATGACCTTCTTACCCGGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACCGGGTAAGAAGGTCATTTCTCGAGAAATGACCTTCTTACCCGGTG-3'

Other clones with same target sequence:

(none)