Construct: shRNA TRCN0000272001

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001099309.1-474s21c1
DNA Barcode:
TGTGGAACTTAACTAACTTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm12887 (666927)
Gene Description:
predicted gene 12887
Transcript:
RefSeq NM_001099309.1 (CURRENT)
Match location:
Position 474 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000272001)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGTGGAACTTAACTAACTTAA
Hairpin Sequence:
5'-CCGG-TGTGGAACTTAACTAACTTAA-CTCGAG-TTAAGTTAGTTAAGTTCCACA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGTGGAACTTAACTAACTTAACTCGAGTTAAGTTAGTTAAGTTCCACATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGTGGAACTTAACTAACTTAACTCGAGTTAAGTTAGTTAAGTTCCACA-3'

Other clones with same target sequence:

(none)