Construct: shRNA TRCN0000272060

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001110250.2-179s21c1
DNA Barcode:
TACTTCTTGACTATGATTCAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm14819 (100039585)
Gene Description:
predicted gene 14819
Transcript:
RefSeq NM_001110250.2 (CURRENT)
Match location:
Position 179 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000272060)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TACTTCTTGACTATGATTCAG
Hairpin Sequence:
5'-CCGG-TACTTCTTGACTATGATTCAG-CTCGAG-CTGAATCATAGTCAAGAAGTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTACTTCTTGACTATGATTCAGCTCGAGCTGAATCATAGTCAAGAAGTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATACTTCTTGACTATGATTCAGCTCGAGCTGAATCATAGTCAAGAAGTA-3'

Other clones with same target sequence:

(none)