Construct: shRNA TRCN0000272071

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001141923.1-447s21c1
DNA Barcode:
TTCATTGGCAGGCTACAATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm9762 (100041766)
Gene Description:
predicted pseudogene 9762; ubiquitin-conjugating enzyme E2D 2 pseudogene
Transcript:
RefSeq NM_001141923.1 (NON-CURRENT)
Match location:
Position 447 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000272071)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTCATTGGCAGGCTACAATAA
Hairpin Sequence:
5'-CCGG-TTCATTGGCAGGCTACAATAA-CTCGAG-TTATTGTAGCCTGCCAATGAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTCATTGGCAGGCTACAATAACTCGAGTTATTGTAGCCTGCCAATGAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTCATTGGCAGGCTACAATAACTCGAGTTATTGTAGCCTGCCAATGAA-3'

Other clones with same target sequence:

(none)